Room 428 at Wilson Hall is permanently closed. Students at Ohio University, the ninth oldest public university in the U.S., are not allowed to so much as enter the dorm room due to the numerous reports of hauntings and dangerous ghostly activity in the room.
Wilson Hall was reportedly built on an Indian burial ground (because that always ends well). Spiritualists claim that the building is located at the center of a pentagram formed by five such ancient cemeteries, lending this geographical position extra powers of either safety or evil.
In the 1970s, a male student died under mysterious circumstances in room 428. Years later, a female student used what she perceived as the room's "energy" to practice rituals such as astral projection, during which the human spirit is said to leave the body and travel about at will. Not long after moving in, this student died as well, reportedly from cutting her own wrists.
Ever since these two student deaths, anyone who lived in the room reported terribly scary occurrences at all hours of the day and night. Objects flew across the room and smashed against the walls. Students heard voices whispering and even shouting even though no one was there. The door would often open and then slam shut of its own accord. Stranger still, demonic faces kept appearing in the wood grain of the room's door. Although the school replaced the door many times, the tortured, ghoulish faces reappeared every time.
Eventually, the room became too frightening and the university feared another student death could result, so they decided to seal the door shut, forever. This is the only known case where a dormitory room has been sealed off in the U.S. to date.
Source: Reddit